BIOL3762 Protein to Proteome - Flinders University

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Overview

In this topic students will learn about the functions of cellular proteins and contemporary approaches that are used for the study and characterisation of proteins. Students will build on concepts introduced at second year level. This topic reviews the process of protein translation, and then examines the post-translational events that affect the structure, function and cellular location of proteins. Traditional techniques for the study of proteins such as protein purification, electrophoresis, western-blotting and enzyme kinetics will be contrasted with more contemporary techniques such as mass spectrometry for identification of entire proteomes and computer based approaches for evaluating protein structure and function.

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Aims

This topic aims to:

Provide an understanding of the processes that regulate and control the function of cellular proteins with a particular emphasis on how protein structure relates to function
Familiarize students with laboratory and bioinformatic techniques that are used to identify and characterise both individual proteins and entire proteomes
Further develop students' scientific skills for work in a biochemistry or molecular biology laboratory
Further develop students' generic skills in the areas of accurate record keeping, creativity, critical thinking, problem-solving, independence, teamwork, and good written and oral communication

Learning outcomes

On completion of this topic you will be expected to be able to:

Examine protein structure, localisation, interaction, regulation, signalling and turnover

Design experimental strategies to answer research questions about protein structure, function and regulation

Combine theoretical and practical skills in a laboratory setting

Manage experimental data, calculations and record-keeping

Draw conclusions from scientific data

Write scientific text

Assessments

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Requisites information

Pre-requisites:

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Handbook

Protein to Proteome

BIOL3762